Gene: Sopen07g030800

General Information

Description UBC35/UBC13A encodes a protein that may play a role in DNA damage responses and error-free post-replicative DNA repair by participating in lysine-63-based polyubiquitination reactions. UBC35/UBC13A can form diubiquitin and triubiquitin chains in combination with MMZ1,2,3,4/(UEV1A,B,C,D) in vitro. It can also functionally complement an mms2 ubc13 mutation in budding yeast by increasing the double mutants viability in the presence of the DNA damaging agent MMS, when it is co-expressed with MMZ / UEV1 genes. A wild type phenotype is restored with MMZ3/UEV1C and MMZ4/UEV1D, but only partial complementation is achieved with MMZ1/UEV1A or MMZ2/UEV1B. | ubiquitin-conjugating enzyme 35 (UBC35) | FUNCTIONS IN: ubiquitin-protein ligase activity, protein binding | INVOLVED IN: root epidermal cell differentiation, response to cadmium ion, postreplication repair, response to iron ion, ubiquitin-dependent protein catabolic process | LOCATED IN: UBC13-MMS2 complex | EXPRESSED IN: male gametophyte, cultured cell, pollen tube | EXPRESSED DURING: L mature pollen stage, M germinated pollen stage | CONTAINS InterPro DOMAIN/s: Ubiquitin-conjugating enzyme/RWD-like , Ubiquitin-conjugating enzyme, E2 | BEST Arabidopsis thaliana protein match is: ubiquitin-conjugating enzyme 36

GO term	Evidence(s)	Provider(s)	Description	Source(s)
GO:0010053	ISO	PLAZA Integrative Orthology	root epidermal cell differentiation	AT1G78870
GO:0010039	ISO	PLAZA Integrative Orthology	response to iron ion	AT1G78870
GO:0046686	ISO	PLAZA Integrative Orthology	response to cadmium ion	AT1G78870
GO:0006301	ISO	PLAZA Integrative Orthology	postreplication repair	AT1G78870
GO:0006511	ISO	PLAZA Integrative Orthology	ubiquitin-dependent protein catabolic process	AT1G78870

GO term	Evidence(s)	Provider(s)	Description	Source(s)
GO:0004842	ISO	PLAZA Integrative Orthology	ubiquitin-protein transferase activity	AT1G78870
GO:0005515	ISO	PLAZA Integrative Orthology	protein binding	AT1G78870

GO term	Evidence(s)	Provider(s)	Description	Source(s)
GO:0031372	ISO	PLAZA Integrative Orthology	UBC13-MMS2 complex	AT1G78870

Experimental Evidence

Computational Reviewed Evidence

Electronic Evidence

InterPro	Description
IPR016135	Ubiquitin-conjugating enzyme/RWD-like
IPR000608	Ubiquitin-conjugating enzyme E2

Mapman id	Description
19.2.2.1.3	Protein homeostasis.ubiquitin-proteasome system.ubiquitin-fold protein conjugation.ubiquitin conjugation (ubiquitylation).ubiquitin-conjugating E2 protein (UBC)