Gene
- Public Gene Name
- Y32H12A.4
- Sequence Name
- Y32H12A.4
- WB ID
- WBGene00021312
- Description
- Y32H12A.4 encodes an ortholog of various protein phosphatase 1 (PP1) regulatory (inhibitor) subunit 2 proteins; the phosphatase inhibitory activity of recombinant Y32H12A.4 protein has been validated in vitro, with an IC50 of 5 nM versus purified PP1 catalytic subunit; Y32H12A.4 has anomalously low mobility in SDS-PAGE, consistent with the hypothesis that it (like its orthologs) is a naturally disordered protein that uses native flexibility to bind its substrates; Y32H12A.4 is efficiently phosphorylated in vitro by casein kinase II or CDC2/cyclinB, but not by glycogen synthase kinase-3; Y32H12A.4 is required for embryonic development in mass RNAi assays.
