InterPro domain: IPR045075

This entry represents a group of pre-mRNA-processing factors, including Syf1/Clf1 from budding yeasts and protein crooked neck (CRN) from fruit flies. Syf1 acts as a component of the NTC complex (or Prp19-associated complex), associates to the spliceosome to mediate conformational rearrangement or to stabilize the structure of the spliceosome after U4 snRNA dissociation, which leads to spliceosome maturation [ 1 , 2 ]. Clf1 is a splicing factor that are part of at least two multisubunit protein complexes, a small nuclear RNA-free structure similar to what was reported as the Prp19 complex and an RNP structure that contains the U2, U5, and U6 small nuclear RNAs [ 3 ]. Human CRN-like protein is also implicated in pre-mRNA splicing [ 4 . The crn mutation in Drosophila has been shown to cause abnormalities in the central and peripheral nervous systems [ 5 ].

1. Genetic and physical interactions between factors involved in both cell cycle progression and pre-mRNA splicing in Saccharomyces cerevisiae. Genetics 156, 1503-17
2. Functional analyses of interacting factors involved in both pre-mRNA splicing and cell cycle progression in Saccharomyces cerevisiae. RNA 6, 1565-72
3. The Clf1p splicing factor promotes spliceosome assembly through N-terminal tetratricopeptide repeat contacts. J. Biol. Chem. 278, 7875-83
4. Crooked neck is a component of the human spliceosome and implicated in the splicing process. Biochim Biophys Acta 1576, 287-97
5. A novel rat orthologue and homologue for the Drosophila crooked neck gene in neural stem cells and their immediate descendants. J Biochem 133, 615-23