InterPro domain: IPR040398

The Ccr4-Not complex is a global regulator of gene expression that is conserved from yeast to human. It affects genes positively and negatively and is thought to regulate transcription factor IID function. In Saccharomyces cerevisiae, it exists in two prominent forms and consists of at least nine core subunits: the five Not proteins (Not1 to Not5), Caf1, Caf40, Caf130 and Ccr4 [ 1 ]. The Ccr4-Not complex regulates many different cellular functions, including RNA degradation and transcription initiation. It may be a regulatory platform that senses nutrient levels and stress [ 2 ]. Caf1 and Ccr4, are directly involved in mRNA deadenylation, and Caf1p is associated with Dhh1, a putative RNA helicase thought to be a component of the decapping complex [ 3 ]. Pop2, a component of the Ccr4-Not complex, functions as a deadenylase [ 4 ].

This entry includes Not1 (also known as Cdc39) and its homologues.

1. Isolation and characterization of human orthologs of yeast CCR4-NOT complex subunits. Nucleic Acids Res. 28, 809-17
2. Global control of gene expression in yeast by the Ccr4-Not complex. Gene 313, 1-16
3. Interaction between Not1p, a component of the Ccr4-not complex, a global regulator of transcription, and Dhh1p, a putative RNA helicase. J. Biol. Chem. 277, 2835-42
4. Biophysical and biochemical characterization of recombinant human Pop2 deadenylase. Protein Expr. Purif. 60, 46-52