InterPro domain: IPR037381

General Information

  • Identifier IPR037381
  • Description E3 ubiquitin-protein ligase RFWD3

Abstract

RFWD3 is a RING-type E3 ubiquitin-protein ligase required for the repair of DNA interstrand cross-links (ICL) in response to DNA damage [ 1 , 2 , 3 ].

During ICL repair, single-stranded DNA (ssDNA) is generated. The trimeric ssDNA binding protein complex RPA coats the ssDNA. Subsequently, RAD51 is loaded onto RPA-bound ssDNA and catalyzes the critical activity in homologous recombination (HR). RFWD3 polyubiquitinates both RPA and RAD5, which increases their local turnover in DNA damage-induced foci to facilitate HR [ 4 ]. RFWD3-mediated ubiquitination of RPA has been shown to be essential for HR [ 4 ].


1. RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol. Cell 66, 622-634.e8
2. RING finger and WD repeat domain 3 (RFWD3) associates with replication protein A (RPA) and facilitates RPA-mediated DNA damage response. J. Biol. Chem. 286, 22314-22
3. E3 ligase RFWD3 participates in replication checkpoint control. J. Biol. Chem. 286, 22308-13
4. RFWD3-Dependent Ubiquitination of RPA Regulates Repair at Stalled Replication Forks. Mol. Cell 60, 280-93

Species distribution

Gene table