InterPro domain: IPR033270

This entry represents the VPRBP/DCAF1 family, whose members include protein VPRBP from mammals [ 1 ], protein mahjong form fruit flies [ 2 ] and DCAF1 from worms and plants [ 3 ]. They are the substrate recognition component of the E3 ubiquitin-protein ligase complex.

Human VPRBP is part of the CUL4A-RBX1-DDB1-DCAF1/VPRBP complex that mediates ubiquitination and proteasome-dependent degradation of proteins [ 4 ]. VPRBP also has kinase activity and is capable of phosphorylating histone H2A on threonine 120 (H2AT120p) in a nucleosomal context [ 5 ].

1. Molecular architecture and assembly of the DDB1-CUL4A ubiquitin ligase machinery. Nature 443, 590-3
2. Involvement of Lgl and Mahjong/VprBP in cell competition. PLoS Biol. 8, e1000422
3. Arabidopsis DDB1-CUL4 ASSOCIATED FACTOR1 forms a nuclear E3 ubiquitin ligase with DDB1 and CUL4 that is involved in multiple plant developmental processes. Plant Cell 20, 1437-55
4. EZH2 generates a methyl degron that is recognized by the DCAF1/DDB1/CUL4 E3 ubiquitin ligase complex. Mol. Cell 48, 572-86
5. VprBP has intrinsic kinase activity targeting histone H2A and represses gene transcription. Mol. Cell 52, 459-67