InterPro domain: IPR001764

O-Glycosyl hydrolases ( 3.2.1. ) are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families [ 1 , 2 ]. This classification is available on the CAZy (CArbohydrate-Active EnZymes) website.

Glycoside hydrolase family 3 GH3 comprises enzymes with a number of known activities; beta-glucosidase ( 3.2.1.21 ); beta-xylosidase ( 3.2.1.37 ); N-acetyl beta-glucosaminidase ( 3.2.1.52 ); glucan beta-1,3-glucosidase ( 3.2.1.58 ); cellodextrinase ( 3.2.1.74 ); exo-1,3-1,4-glucanase ( 3.2.1 ).These enzymes are two-domain globular proteins that are N-glycosylated at three sites [ 3 ]. This domain is often N-terminal to the glycoside hydrolase family 3, C-terminal domain IPR002772 .

1. Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases. Proc. Natl. Acad. Sci. U.S.A. 92, 7090-4
2. Structures and mechanisms of glycosyl hydrolases. Structure 3, 853-9
3. Three-dimensional structure of a barley beta-D-glucan exohydrolase, a family 3 glycosyl hydrolase. Structure 7, 179-90