InterPro domain: IPR045145

This entry includes chromatin assembly factor 1 subunit CHAF1B (also known as p60 subunit) from animals, and its homologues such as Cac2 from yeasts and FAS from Arabidopsis. They are a component of the chromatin assembly factor complex (CAF-1), an evolutionarily conserved heterotrimeric chaperone comprised of p150, p60 and p48 subunits, mediates DNA synthesis-coupled chromatin assembly in S-phase. It is also involved in chromatin assembly following DNA repair [ 1 , 2 ]. In plants, the loss of activity of the CAF1 pathway causes delay and arrest of the cell cycle during pollen development [ 3 ]. The CAF1 complex is also required for stable maintenance of telomeres and 45S rDNA in Arabidopsis [ 4 ].

1. Recruitment of phosphorylated chromatin assembly factor 1 to chromatin after UV irradiation of human cells. J Cell Biol 143, 563-75
2. Structure of a human ASF1a-HIRA complex and insights into specificity of histone chaperone complex assembly. Nat. Struct. Mol. Biol. 13, 921-9
3. Chromatin assembly factor 1 regulates the cell cycle but not cell fate during male gametogenesis in Arabidopsis thaliana. Development 135, 65-73
4. Dysfunction of chromatin assembly factor 1 induces shortening of telomeres and loss of 45S rDNA in Arabidopsis thaliana. Plant Cell 22, 2768-80