InterPro domain: IPR045116

Polyribonucleotide 5-hydroxyl-kinase Clp1 is a component of the cleavage factor IA (CF IA) complex, which is involved in the endonucleolytic cleavage during polyadenylation-dependent pre-mRNA 3'-end formation [ 1 ]. It is involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation [ 2 ]. Human Clp1 can also function as a tRNA splicing enzyme [ 2 ].

This entry includes Clp1 and Grc3/NOL9.Grc3 and its homologue, NOL9, are polynucleotide 5'-kinases involved in rRNA processing [ 3 , 4 ].

1. Five subunits are required for reconstitution of the cleavage and polyadenylation activities of Saccharomyces cerevisiae cleavage factor I. Proc. Natl. Acad. Sci. U.S.A. 98, 6080-5
2. Human RNA 5'-kinase (hClp1) can function as a tRNA splicing enzyme in vivo. RNA 14, 1737-45
3. Role of the RNA/DNA kinase Grc3 in transcription termination by RNA polymerase I. EMBO Rep 11, 758-64
4. Nol9 is a novel polynucleotide 5'-kinase involved in ribosomal RNA processing. EMBO J 29, 4161-71