InterPro domain: IPR045091

This entry includes Mad2 and Mad2-like proteins, including Mad2/Rev7 from yeasts and MAD2A/B from animals. Mad2 is a central component of the spindle assembly checkpoint, which is a feedback control that prevents cells with incompletely assembled spindles from leaving mitosis [ 1 , 2 ]. Rev7 (also known as MAD2L2 or MAD2B) is an accessory subunit of DNA polymerase zeta [ 3 ]. It also acts as an interaction module in a broad array of cellular pathways, including translesion DNA synthesis, cell cycle control, and nonhomologous end joining [ 4 ].

1. The mitotic feedback control gene MAD2 encodes the alpha-subunit of a prenyltransferase. Nature 366, 82-4
2. Two complexes of spindle checkpoint proteins containing Cdc20 and Mad2 assemble during mitosis independently of the kinetochore in Saccharomyces cerevisiae. Eukaryotic Cell 4, 867-78
3. Saccharomyces cerevisiae polymerase zeta functions in mitochondria. Genetics 172, 2683-8
4. REV7 has a dynamic adaptor region to accommodate small GTPase RAN/Shigella IpaB ligands, and its activity is regulated by the RanGTP/GDP switch. J Biol Chem 294, 15733-15742