InterPro domain: IPR044152

This entry represents a group of NADPH dehydrogenases, including NamA (also known as YqjM) from Bacillus subtilis. YqjM is a member of the Old Yellow Enzyme (OYE) family of flavin oxidoreductases. Similar to other OYE members, YqjM binds the FMN cofactor non-covalently and reduces the flavin in the reductive half-reaction at the expense of NADPH. It transfers electrons from the reduced flavin to the double bond of a variety of alpha, beta-unsaturated carbonyl compounds and accepts also electrophilic xenobiotics such as nitroglycerin, N-ethylmaleimide, and trinitrotoluene as substrates for the oxidative half-reaction [ 1 , 2 ].

This entry also includes AfvA from Aspergillus flavus. AfvA is part of the gene cluster that mediates the biosynthesis of aflavarin, a bicoumarin that exhibits anti-insectan activity against the fungivorous beetle C.hemipterus [ 3 ].

1. Characterization of YqjM, an Old Yellow Enzyme homolog from Bacillus subtilis involved in the oxidative stress response. J. Biol. Chem. 278, 19891-7
2. The 1.3 A crystal structure of the flavoprotein YqjM reveals a novel class of Old Yellow Enzymes. J. Biol. Chem. 280, 27904-13
3. Transcriptome Analysis of Aspergillus flavus Reveals veA-Dependent Regulation of Secondary Metabolite Gene Clusters, Including the Novel Aflavarin Cluster. Eukaryotic Cell 14, 983-97