InterPro domain: IPR042487

RuvB-like helicase 2 (RUVBL2 or TIP49b; 3.6.4.12 ) has single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. It forms a homohexamer which is critical for ATP hydrolysis, and forms a dodecamer with RUVBL1 [ 1 , 2 ]. RUVBL2 is an essential cofactor for oncogenic transformation by c-Myc [ 3 ]. It is a component of the NuA4 histone acetyltransferase [ 4 ], BAF53 [ 5 ], MLL1/MLL [ 6 ], INO80 [ 7 ] and R2TP [ 8 ] complexes.

This entry represents the second domain of RuvBL1 and RUVB2 which is involved in DNA/RNA binding [ 9 ].RuvBL1 is a ubiquitously expressed protein that plays important roles in chromatin remodeling, transcription, DNA repair, and apoptosis. The significant evolutionary conservation of RuvBL1 from yeast to man strongly suggests that it mediates important cellular functions. RuvBL1 belongs to a large family of ATPases known as AAA proteins (ATPases associated with diverse cellular activities) which generally form hexamers. RuvBL1 is the eukaryotic homologue of the bacterial DNA-dependent ATPase and helicase RuvB [ 10 ].

1. TIP49b, a new RuvB-like DNA helicase, is included in a complex together with another RuvB-like DNA helicase, TIP49a. J. Biol. Chem. 274, 22437-44
2. Dodecameric structure and ATPase activity of the human TIP48/TIP49 complex. J. Mol. Biol. 366, 179-92
3. An ATPase/helicase complex is an essential cofactor for oncogenic transformation by c-Myc. Mol. Cell 5, 321-30
4. Identification of new subunits of the multiprotein mammalian TRRAP/TIP60-containing histone acetyltransferase complex. J. Biol. Chem. 278, 42733-6
5. BAF53 forms distinct nuclear complexes and functions as a critical c-Myc-interacting nuclear cofactor for oncogenic transformation. Mol. Cell. Biol. 22, 1307-16
6. Physical association and coordinate function of the H3 K4 methyltransferase MLL1 and the H4 K16 acetyltransferase MOF. Cell 121, 873-85
7. A mammalian chromatin remodeling complex with similarities to the yeast INO80 complex. J. Biol. Chem. 280, 41207-12
8. CK2 phospho-dependent binding of R2TP complex to TEL2 is essential for mTOR and SMG1 stability. Mol. Cell 39, 839-50
9. Crystal structure of the human AAA+ protein RuvBL1. J. Biol. Chem. 281, 38918-29