InterPro domain: IPR042203

This superfamily represents the C-terminal domain of leucyl/phenylalanyl-tRNA-protein transferases (L/F-transferases).

L/F-transferase is an N-end rule pathway enzyme, involved in the transfer of Leu and Phe from aminoacyl-tRNAs to exposed N-terminal Arg or Lys residues of acceptor proteins [ 1 ]. This C-terminal domain has a highly conserved fold, observed in the GCN5-related N-acetyltransferase (GNAT) family.The interface of the N-terminal and C-terminal domains forms a large central cavity, with the characteristic positive charge contributed by the corner of the cleft on the C-terminal domain surface, suggesting that they probably complement the negatively charged phosphate backbone of the substrate aminoacyl-tRNA [ 2 ].

1. Protein-based peptide-bond formation by aminoacyl-tRNA protein transferase. Nature 449, 867-71
2. The crystal structure of leucyl/phenylalanyl-tRNA-protein transferase from Escherichia coli. Protein Sci. 16, 528-34