InterPro domain: IPR041707

This group consists of eukaryotic pseudouridine synthases similar to S. cerevisiae Pus3p. Pseudouridine synthases catalyze the isomerization of specific uridines in an RNA molecule to pseudouridines (5-ribosyluracil, psi) [ 1 ]. No cofactors are required. S. cerevisiae Pus3p makes psi38 and psi39 in tRNAs [ 2 ]. Mouse Pus3 has been shown to makes psi38 and, possibly also psi 39, in tRNAs [ 3 ]. Psi38 and psi39 are highly conserved in tRNAs from eubacteria, archea and eukarya [ 4 ].

1. Pseudouridine in RNA: what, where, how, and why. IUBMB Life 49, 341-51
2. Characterization of yeast protein Deg1 as pseudouridine synthase (Pus3) catalyzing the formation of psi 38 and psi 39 in tRNA anticodon loop. J. Biol. Chem. 273, 1316-23
3. Pseudouridine synthase 3 from mouse modifies the anticodon loop of tRNA. Biochemistry 39, 12723-30
4. The structural basis for tRNA recognition and pseudouridine formation by pseudouridine synthase I. Nat. Struct. Biol. 7, 23-7