InterPro domain: IPR040466

Nkap is a highly conserved protein which functions as a transcriptional repressor on Notch target genes. It is required for T cell development and acquisition of functional competency [ 1 , 2 , 3 , 4 ], in transcriptional repression and maintenance and survival of adult hematopoietic stem cells [ 5 ], it also plays a role in RNA splicing and processing [ 5 , 5 ]. MSA2, the Nkap human orthologue in Arabidopsis thaliana, is involved in rRNA synthesis [ 6 ]. Nkap is a key regulator of mitosis, and its dysregulation might contribute to tumorigenesis by causing chromosomal instability [ 7 ]. Nkap promotes the proliferation, migration and invasion of Ewing sarcoma cells, through the AKT signalling pathway [ 8 ].

NKAP is divided into approximately three domains, N-terminal RS repeats, a highly basic middle domain and the C-terminal DUF 926 [ 9 , 9 ].

1. NKAP is a transcriptional repressor of notch signaling and is required for T cell development. Immunity 30, 696-707
2. NKAP is required for T cell maturation and acquisition of functional competency. J. Exp. Med. 208, 1291-304
3. The transcriptional repressor NKAP is required for the development of iNKT cells. Nat Commun 4, 1582
4. NKAP is a novel RS-related protein that interacts with RNA and RNA binding proteins. Nucleic Acids Res. 42, 3177-93
5. The C-Terminal SynMuv/DdDUF926 Domain Regulates the Function of the N-Terminal Domain of DdNKAP. PLoS ONE 11, e0168617
6. Arabidopsis MAS2, an Essential Gene That Encodes a Homolog of Animal NF-κ B Activating Protein, Is Involved in 45S Ribosomal DNA Silencing. Plant Cell 27, 1999-2015
7. SUMOylated NKAP is essential for chromosome alignment by anchoring CENP-E to kinetochores. Nat Commun 7, 12969
8. NKAP functions as an oncogene in Ewing sarcoma cells partly through the AKT signaling pathway. Exp Ther Med 18, 3037-3045