InterPro domain: IPR040258

Proteins in this entry are subunits the FACT complex; the FACT complex is a stable heterodimer in Saccharomyces cerevisiae comprising Spt16 and Pob3. The complex plays a role in transcription initiation and promotes binding of TATA-binding protein (TBP) to a TATA box in chromatin [ 1 ]; it also facilitates RNA polymerase II transcription elongation through nucleosomes by destabilising and then reassembling nucleosome structure [ 2 , 3 ].

The proteins in this entry are non-peptidase homologues belonging to MEROPS peptidase family M24 (clan MG).

1. The yeast FACT complex has a role in transcriptional initiation. Mol. Cell. Biol. 25, 5812-22
2. Defects in SPT16 or POB3 (yFACT) in Saccharomyces cerevisiae cause dependence on the Hir/Hpc pathway: polymerase passage may degrade chromatin structure. Genetics 162, 1557-71
3. FACT facilitates transcription-dependent nucleosome alteration. Science 301, 1090-3