InterPro domain: IPR039757

Proteins in this entry contain at least an N-terminal PUA (PseudoUridine synthase and Archaeosine transglycosylase) domain and a C-terminal SUI1 domain. Proteins with this architecture include:

• Eukaryotic translation initiation factor 2D (EIF2D; formerly and erroneously known as ligatin): a factor that initiates translation by delivering tRNA to the P-site, where the initiating AUG codon binds, of the eukaryotic ribosome in a GTP-independent manner [ 1 ]. EIF2D recruits aminoacylated initiator tRNA (Met-tRNA(Met)(i)) to 40S/mRNA complexes and promotes release of deacylated tRNA and mRNA from recycled 40S subunits after ABCE1-mediated dissociation of post-termination ribosomes [ 2 ].
• Translation machinery-associated protein 64 (Tma64) from the yeast Saccharomyces cerevisiae , which is uncharacterized but also interacts with the 40S ribosomal subunit [ 3 ].

1. GTP-independent tRNA delivery to the ribosomal P-site by a novel eukaryotic translation factor. J. Biol. Chem. 285, 26779-87
2. Activities of Ligatin and MCT-1/DENR in eukaryotic translation initiation and ribosomal recycling. Genes Dev. 24, 1787-801
3. Systematic identification and functional screens of uncharacterized proteins associated with eukaryotic ribosomal complexes. Genes Dev. 20, 1294-307