InterPro domain: IPR039171

General Information

  • Identifier IPR039171
  • Description Pre-mRNA-splicing factor Cwc2/Slt11
  • Number of genes 237
  • Gene duplication stats Loading...
  • Associated GO terms GO:0003723  

Abstract

This entry contains proteins involved in the first step of pre-mRNA splicing to remove introns. Splicing is performed by the spliceosome, which is a complex of snRNAs U1, U2, U4, U5, and U6 and proteins such as pre-mRNA-processing factor 19 (Prp19). The yeast protein Cwc2 contains motifs known to bind RNA, a zinc finger and an RNA recognition motif. Because mutations in the gene are lethal and lead to accumulation of pre-mRNA, and reduced levels of U1, U4, U5 and U6 snRNAs and U4/U6 snRNA complex levels, Cwc2 has been proposed to link the Prp19 complex to the spliceosome during pre-mRNA splicing [ 1 ]. Because Cwc2 is at the spliceosome catalytic centre, it induces an active conformation of the spliceosome's catalytic RNA elements [ 2 ]. Cwc2 is a component of the CWC complex [ 3 ]. The human homologue, known as RBM22 [ 4 ], also translocates the protein SLU7 from the nucleus to the cytoplasm or vice versa in the case of the calcium-binding protein PDCD6 [ 4 ].

Also included in this entry is the pre-mRNA-splicing factor Slt11 (also known as Ecm2) [ 5 ].


1. The RNA binding protein Cwc2 interacts directly with the U6 snRNA to link the nineteen complex to the spliceosome during pre-mRNA splicing. Nucleic Acids Res. 37, 4205-17
2. Cwc2 and its human homologue RBM22 promote an active conformation of the spliceosome catalytic centre. EMBO J. 31, 1591-604
3. Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs. Mol. Cell. Biol. 22, 2011-24
4. Nuclear translocation of the calcium-binding protein ALG-2 induced by the RNA-binding protein RBM22. Biochim. Biophys. Acta 1763, 1335-43
5. Synthetic lethality of yeast slt mutations with U2 small nuclear RNA mutations suggests functional interactions between U2 and U5 snRNPs that are important for both steps of pre-mRNA splicing. Mol. Cell. Biol. 18, 2055-66

Species distribution

Gene table

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