InterPro domain: IPR038499

The BRO1 domain is a protein domain of ~390 residues in length. It occurs in a number of eukaryotic proteins, such as yeast BRO1 and human PDCD6IP/Alix, which are involved in protein targeting to the vacuole or lysosome. The BRO1 domain of fungal and mammalian proteins binds with multivesicular body components (ESCRT-III proteins) such as yeast Snf7 and mammalian CHMP4b, and can function to target BRO1 domain-containing proteins to endosomes [ 1 , 2 , 3 ].

The BRO1 domain has a boomerang shape composed of 14 alpha-helices and 3 beta-sheets. It contains a TPR-like substructure in the central part [ 4 ]. The C terminus is less conserved.

1. Structural basis for endosomal targeting by the Bro1 domain. Dev. Cell 8, 937-47
2. Structure and function of human Vps20 and Snf7 proteins. Biochem. J. 377, 693-700
3. Mutational analysis of the pH signal transduction component PalC of Aspergillus nidulans supports distant similarity to BRO1 domain family members. Genetics 171, 393-401