InterPro domain: IPR038108

This entry represents the DEUBiquitinase ADaptor (DEUBAD) domain from RPN13 (ADRM1), located at its C-terminal, that binds to the UCH37-like domain (ULD) of the deubiquitinase UCH37 [ 1 , 2 , 3 , 4 ]. This interaction is required for DUB activity activation.

Its structure consists of eight alpha-helices that form a helical bundle surrounding a compact hydrophobic core [ 5 ]. It has a modular architecture with the core formed by helices 1-4, primarily responsible for binding to ULD, and accessory elements that lead to full activation, or inhibition, of the UCH activity [ 5 , 5 ].

1. Proteasome recruitment and activation of the Uch37 deubiquitinating enzyme by Adrm1. Nat. Cell Biol. 8, 994-1002
2. Structure of proteasome ubiquitin receptor hRpn13 and its activation by the scaffolding protein hRpn2. Mol. Cell 38, 404-15
3. Mechanism of UCH-L5 activation and inhibition by DEUBAD domains in RPN13 and INO80G. Mol Cell 57, 887-900
4. Mechanism of the Rpn13-induced activation of Uch37. Protein Cell 5, 616-30
5. Structural Basis for the Activation of the Deubiquitinase Calypso by the Polycomb Protein ASX. Structure 27, 528-536.e4