InterPro domain: IPR038090
General Information
- Identifier IPR038090
- Description DNA replication factor Cdt1, C-terminal WH domain superfamily
- Number of genes 284
- Gene duplication stats Loading...
Abstract
In eukaryotic replication licensing, Cdt1 plays a key role by recruiting the MCM2-7 complex onto the origin of chromosome. This entry represents the C-terminal domain of Cdt1 which is the most conserved region of Cdt1, essential for licensing and directly interacts with the MCM2-7 complex [ 1 ]. This region contains a WHD (winged helix domain), a typical DNA recognition motif [ 2 ]. The winged-helix fold is conserved throughout several other licensing factors, suggesting that they evolved from a common ancestor. In mouse Cdt1, the main role of the WH motif is to interact with MCM2-7 rather than with the DNA, as other winged-helix domains.
1. Structure of the Cdt1 C-terminal domain: conservation of the winged helix fold in replication licensing factors. Protein Sci. 18, 2252-64
2. Structure and mutagenesis studies of the C-terminal region of licensing factor Cdt1 enable the identification of key residues for binding to replicative helicase Mcm proteins. J Biol Chem 285, 15931-40