InterPro domain: IPR037151
General Information
- Identifier IPR037151
- Description Alpha-ketoglutarate-dependent dioxygenase AlkB-like superfamily
- Number of genes 1346
- Gene duplication stats Loading...
Abstract
AlkB is a DNA repair enzyme that removes methyl adducts and some larger alkylation lesions from endocyclic positions on purine and pyrimidine bases. It is a dioxygenase that requires molecular oxygen, alpha-ketoglutarate and iron [ 1 , 2 ]. The catalytic core of AlkB is homologous to other Fe-2OG dioxygenases [ 3 ]. Two discrete global conformations have been observed for AlkB, differing in accessibility of a putative oxygen-diffusion tunnel [ 4 ].
1. AlkB-mediated oxidative demethylation reverses DNA damage in Escherichia coli. Nature 419, 178-82
2. Iron-catalysed oxidation intermediates captured in a DNA repair dioxygenase. Nature 468, 330-3
3. Structural studies on 2-oxoglutarate oxygenases and related double-stranded beta-helix fold proteins. J. Inorg. Biochem. 100, 644-69
4. Enzymological and structural studies of the mechanism of promiscuous substrate recognition by the oxidative DNA repair enzyme AlkB. Proc. Natl. Acad. Sci. U.S.A. 106, 14315-20