InterPro domain: IPR036104
General Information
- Identifier IPR036104
- Description Bifunctional nuclease superfamily
- Number of genes 314
- Gene duplication stats Loading...
- Associated GO terms GO:0004518
Abstract
The bifunctional nuclease (BFN) domain is specific to bacteria and plantorganisms. It has both RNase and DNase activities [ 1 ].
The dimer of the BFN domain forms a wedge, each monomer being a basictriangular shape. The BFN domain is composed of an eight-stranded, distortedbeta-sheet consisting of a four-stranded, antiparallel beta-sheet (B1, B2, B3,B8), and a four-stranded mixed beta-sheet (B4, B5, B6, B7). The sheets areintercalated by three short alpha-helices (H1, H3, H4), while a longer alpha-helix (H2) forms the central core of the dimer interface [ 2 ].Some proteins known to contain a BFN domain are listed below:
- Thermotoga maritima TM0160, a conserved hypothetical protein.
- Plant bifunctional nuclease in basal defense response (BBD) proteins, regulators in abscisic acid (ABA)-mediated defense responses.
This entry represents the BFN superfamily.
1. Novel bifunctional nucleases, OmBBD and AtBBD1, are involved in abscisic acid-mediated callose deposition in Arabidopsis. Plant Physiol. 152, 1015-29
2. On the use of DXMS to produce more crystallizable proteins: structures of the T. maritima proteins TM0160 and TM1171. Protein Sci. 13, 3187-99