InterPro domain: IPR035650

This domain is found in the C terminus of ribosomal protection proteins Tet(M) and Tet(O), and related proteins. This domain has homology to the C-terminal domains of the elongation factors EF-G and EF-2. Tet(M) and Tet(O) catalyze the release of tetracycline (Tc) from the ribosome in a GTP-dependent manner, thereby mediating Tc resistance [ 1 , 2 , 3 ]. Tcs are broad-spectrum antibiotics. Typical Tcs bind to the ribosome and inhibit the elongation phase of protein synthesis, by inhibiting the occupation of site A by aminoacyl-tRNA [ 4 , 5 ].

1. Tet(M)-promoted release of tetracycline from ribosomes is GTP dependent. null 178, 3246-51
2. The tetracycline resistance protein Tet(o) perturbs the conformation of the ribosomal decoding centre. null 45, 1463-72
3. Localization of the ribosomal protection protein Tet(O) on the ribosome and the mechanism of tetracycline resistance. null 7, 1037-45
4. Ribosomal protection proteins and their mechanism of tetracycline resistance. Mol. Microbiol. 47, 3675-81
5. Tetracycline resistance mediated by ribosomal protection. Mol. Cell 40, 1-5