InterPro domain: IPR035434
General Information
- Identifier IPR035434
- Description Glutamate--cysteine ligase, bacteria and plant
- Number of genes 215
- Gene duplication stats Loading...
- Associated GO terms GO:0006750 GO:0004357
Abstract
This entry represents glutamate--cysteine ligase (GCL) from bacteria and plants, including EgtA from Actinobacteria and chloroplastic GCL from Arabidopsis.
EgtA resembles the glutamate--cysteine ligase of the two-step pathway for glutathione (GSH) biosynthesis, but instead are involved in the biosynthesis of the histidine-derived thiol, ergothioneine (EGT). Successful in vitro reconstitution of EGT biosynthesis using EgtBCDE and gamma-L-glutamyl-L-cysteine suggests that this enzyme is a bone fide glutamate--cysteine ligase [ 1 ].
Arabidopsis GCL, known as GSH1, is involved in step 1 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate. It may play a role in disease resistance [ 2 ].
1. In vitro reconstitution of Mycobacterial ergothioneine biosynthesis. J. Am. Chem. Soc. 132, 6632-3
2. Evidence for a direct link between glutathione biosynthesis and stress defense gene expression in Arabidopsis. Plant Cell 16, 2448-62