InterPro domain: IPR035299

This entry represents non-ATPase regulatory subunit 14 of the 26S proteasome (gene name PSMD14, Rpn11 or POH1), a component of the 19S cap [ 1 ]. PSMD14 is a metallopeptidase and a deubiquitinating hydrolase (MEROPS identifier M67.001), releasing 'Lys-63'-linked polyubiquitin chains from the polyubiquitinated protein substrates of the proteasome [ 2 , 3 , 4 ]. PSMD14 also processes polyubiquitin from conjugates generated as a response to double-strand DNA breaks [ 5 ].

1. Structure of the human 26S proteasome at a resolution of 3.9 A. Proc. Natl. Acad. Sci. U.S.A. 113, 7816-21
2. Disassembly of Lys11 and mixed linkage polyubiquitin conjugates provides insights into function of proteasomal deubiquitinases Rpn11 and Ubp6. J. Biol. Chem. 290, 4688-704
3. The proteasomal de-ubiquitinating enzyme POH1 promotes the double-strand DNA break response. EMBO J. 31, 3918-34
4. K63-specific deubiquitination by two JAMM/MPN+ complexes: BRISC-associated Brcc36 and proteasomal Poh1. EMBO J. 28, 621-31