InterPro domain: IPR034653

This entry represents the RNA recognition motif (RRM) of SPF45.

SPF45, also termed RNA-binding motif protein 17 (RBM17), is an RNA-binding protein consisting of an unstructured N-terminal region, followed by a G-patch motif and a C-terminal U2AF (U2 auxiliary factor) homology motifs (UHM) that harbours a RNA recognition motif (RRM) and an Arg-Xaa-Phe sequence motif. SPF45 is a bifunctional protein that functions in splicing and DNA repair. It is involved in regulating alternative splicing of the apoptosis regulatory gene FAS (also called CD95). Its UHM binds UHM-ligand motifs (ULMs) present in the 3' splice site-recognizing factors U2AF65, SF1 and SF3b155 [ 1 ]. It binds to the Holliday junction, which is a key DNA intermediate in the homologous-recombination pathway [ 2 , 3 ].

1. U2AF-homology motif interactions are required for alternative splicing regulation by SPF45. Nat. Struct. Mol. Biol. 14, 620-9
2. Drosophila SPF45: a bifunctional protein with roles in both splicing and DNA repair. PLoS Genet. 2, e178
3. Holliday junction-binding activity of human SPF45. Genes Cells 15, 373-83