InterPro domain: IPR034424

This entry represents the RNA recognition motif 2 (RRM2) of G-rich sequence factor 1 (GRSF-1).

GRSF-1 is a cytoplasmic poly(A)+ mRNA binding protein which interacts with RNA in a G-rich element-dependent manner [ 1 ]. It regulates RNA processing in mitochondrial RNA granules [ 2 , 3 ]. It also mediates vial mRNA translation in infected cells [ 4 ]. GRSF-1 contains three potential RNA recognition motifs (RRMs), which are responsible for the RNA binding. In addition, GRSF-1 has two auxiliary domains, an acidic alpha-helical domain and an N-terminal alanine-rich region, that may play a role in protein-protein interactions and provide binding specificity [ 5 , 5 ].

1. GRSF-1: a poly(A)+ mRNA binding protein which interacts with a conserved G-rich element. Nucleic Acids Res. 22, 2334-43
2. GRSF1 regulates RNA processing in mitochondrial RNA granules. Cell Metab. 17, 399-410
3. The mitochondrial RNA-binding protein GRSF1 localizes to RNA granules and is required for posttranscriptional mitochondrial gene expression. Cell Metab. 17, 386-98
4. Regulation of eukaryotic protein synthesis: selective influenza viral mRNA translation is mediated by the cellular RNA-binding protein GRSF-1. Proc. Natl. Acad. Sci. U.S.A. 96, 6694-9
5. Selective translation of eukaryotic mRNAs: functional molecular analysis of GRSF-1, a positive regulator of influenza virus protein synthesis. J. Virol. 76, 10417-26