InterPro domain: IPR034240

This entry represents the RNA recognition motif (RRM) of eIF-3G (Eukaryotic translation initiation factor 3 subunit G) and similar proteins. eIF-3G is the RNA-binding subunit of eIF3, a large multisubunit complex that plays a central role in the initiation of translation by binding to the 40 S ribosomal subunit and promoting the binding of methionyl-tRNAi and mRNA [ 1 , 2 ]. eIF-3G binds 18 S rRNA and beta-globin mRNA, and therefore appears to be a nonspecific RNA-binding protein [ 3 ]. eIF-3G is one of the cytosolic targets and interacts with mature apoptosis-inducing factor (AIF) [ 4 ]. eIF-3G contains one RNA recognition motif (RRM). Proteins containing this motif include yeast eIF3-p33 (also known as Tif35) [ 5 ]. eIF3-p33 is a homologue of vertebrate eIF-3G, and plays an important role in the initiation phase of protein synthesis. It binds both mRNA and rRNA fragments due to an RRM near its C terminus [ 6 ].

1. Translation initiation factor eIF4G-1 binds to eIF3 through the eIF3e subunit. J. Biol. Chem. 281, 22917-32
2. Reconstitution reveals the functional core of mammalian eIF3. EMBO J. 26, 3373-83
3. Binding of eukaryotic initiation factor 3 to ribosomal 40S subunits and its role in ribosomal dissociation and anti-association. RNA 11, 470-86
4. Apoptosis-inducing factor (AIF) inhibits protein synthesis by interacting with the eukaryotic translation initiation factor 3 subunit p44 (eIF3g). FEBS Lett. 580, 6375-83
5. The RNA recognition motif of eukaryotic translation initiation factor 3g (eIF3g) is required for resumption of scanning of posttermination ribosomes for reinitiation on GCN4 and together with eIF3i stimulates linear scanning. Mol. Cell. Biol. 30, 4671-86
6. Characterization of the p33 subunit of eukaryotic translation initiation factor-3 from Saccharomyces cerevisiae. J. Biol. Chem. 274, 8546-53