InterPro domain: IPR034211

This entry represents the RNA recognition motif 2 (RRM2) of PUF60 (also known as FIR). PUF60 is an essential splicing factor that functions as a poly-U RNA-binding protein required to reconstitute splicing in depleted nuclear extracts. Its function is enhanced through interaction with U2 auxiliary factor U2AF65 [ 1 ]. PUF60 also controls human c-myc gene expression by binding and inhibiting the transcription factor far upstream sequence element (FUSE)-binding-protein (FBP), an activator of c-myc promoters [ 2 ]. It has anti-inflammatory properties, playing a role in the host response [ 3 ]. PUF60 contains two central RNA recognition motifs (RRMs) and a C-terminal U2AF (U2 auxiliary factor) homology motifs (UHM) that harbours another RRM and binds to tryptophan-containing linear peptide motifs (UHM ligand motifs, ULMs) in several nuclear proteins [ 4 ].

1. Control of pre-mRNA splicing by the general splicing factors PUF60 and U2AF(65). PLoS ONE 2, e538
2. An essential role of alternative splicing of c-myc suppressor FUSE-binding protein-interacting repressor in carcinogenesis. Cancer Res. 66, 1409-17
3. Evolutionarily conserved regulation of immunity by the splicing factor RNP-6/PUF60. Elife 9
4. Backbone assignment of the UHM domain of Puf60 free and bound to five ligands. Biomol NMR Assign 2, 211-4