InterPro domain: IPR034131

This entry represents the RNA recognition motif 2 of DAZAP1.

DAZAP1, initially identified as a binding partner of Deleted in Azoospermia (DAZ), is a multi-functional ubiquitous RNA-binding protein expressed most abundantly in the testis and is essential for normal cell growth, development, and spermatogenesis [ 1 , 2 ]. DAZAP1 is a shuttling protein whose acetylated form is predominantly nuclear and the nonacetylated form is in cytoplasm [ 3 ]. DAZAP1 also functions as a translational regulator that activates translation in an mRNA-specific manner [ 4 ]. It also interacts with numerous hnRNPs, including hnRNP U, hnRNP U like-1, hnRNPA1, hnRNPA/B, and hnRNP D, suggesting DAZAP1 might associate and cooperate with hnRNP particles to regulate adenylate-uridylate-rich elements (AU-rich element or ARE)-containing mRNAs [ 5 , 5 ]. DAZAP1 contains two N-terminal RNA recognition motifs (RRMs), also termed RBDs (RNA binding domains) or RNPs (ribonucleoprotein domains), and a C-terminal proline-rich domain [ 6 ].

1. Characterization of the mouse Dazap1 gene encoding an RNA-binding protein that interacts with infertility factors DAZ and DAZL. BMC Genomics 2, 6
2. DAZAP1, an RNA-binding protein required for development and spermatogenesis, can regulate mRNA translation. RNA 17, 1282-95
3. A novel nucleocytoplasmic shuttling sequence of DAZAP1, a testis-abundant RNA-binding protein. RNA 12, 1486-93
4. DAZAP1 interacts via its RNA-recognition motifs with the C-termini of other RNA-binding proteins. Biochem. Biophys. Res. Commun. 380, 705-9
5. DAZAP1, an hnRNP protein, is required for normal growth and spermatogenesis in mice. RNA 14, 1814-22