InterPro domain: IPR033757

Pre-mRNA-splicing regulator WTAP (Wilms' tumor 1-associating protein) is a regulatory subunit of the WMM N6-methyltransferase complex, a multiprotein complex that mediates N6-methyladenosine (m6A) formation, which acts as an epitranscriptomic marker [ 1 , 2 ]. It plays a role in the efficiency of mRNA splicing and processing, and mRNA stability [ 3 ]. In Drosophila, the homologous protein pfemale-lethal(2)D has been implicated in alternative splicing regulation of genes involved in sexual determination [ 4 ] and is required for eye development [ 5 ]. FKBP12-interacting protein of 37kDa (AtFIP37, At3g54170), the homologue from Arabidopsis, may also have a role in splicing events [ 6 ]. AtFIP37 interacts with mRNA adenosine methylase (MTA, encoded by At4g10760) in vitro and in vivo [ 7 ].

1. Mammalian WTAP is a regulatory subunit of the RNA N6-methyladenosine methyltransferase. Cell Res. 24, 177-89
2. A METTL3-METTL14 complex mediates mammalian nuclear RNA N6-adenosine methylation. Nat. Chem. Biol. 10, 93-5
3. Wilms' tumor 1-associating protein regulates G2/M transition through stabilization of cyclin A2 mRNA. Proc. Natl. Acad. Sci. U.S.A. 103, 17278-83
4. Biochemical function of female-lethal (2)D/Wilms' tumor suppressor-1-associated proteins in alternative pre-mRNA splicing. J. Biol. Chem. 278, 3040-7
5. The Drosophila Wilms׳ Tumor 1-Associating Protein (WTAP) homolog is required for eye development. Dev. Biol. 390, 170-80
6. The immunophilin-interacting protein AtFIP37 from Arabidopsis is essential for plant development and is involved in trichome endoreduplication. Plant Physiol. 134, 1283-92
7. MTA is an Arabidopsis messenger RNA adenosine methylase and interacts with a homolog of a sex-specific splicing factor. Plant Cell 20, 1278-88