InterPro domain: IPR032974

Dolichol kinase catalyses the CTP-mediated phosphorylation of dolichol in eukaryotic cells, the terminal step in dolichyl monophosphate (Dol-P) biosynthesis de novo. Dol-P serves as a glycosyl carrier lipid in the assembly of N-linked glycoproteins, glycosylphosphatidylinositol anchors, and the C- and O-mannosylation of proteins in the endoplasmic reticulum (ER) of yeast and animal cells [ 1 , 2 , 3 ]. Phytol kinase catalyses the CTP-dependent phosphorylation of phytol to phytylmonophosphate (PMP) and has a role in tocopherol biosynthesis in plants [ 4 , 5 ]. Both of these enzymes belong to the polyprenol kinase family.

1. Saccharomyces cerevisiae sec59 cells are deficient in dolichol kinase activity. Proc. Natl. Acad. Sci. U.S.A. 89, 7013-6
2. Expression and characterization of a human cDNA that complements the temperature-sensitive defect in dolichol kinase activity in the yeast sec59-1 mutant: the enzymatic phosphorylation of dolichol and diacylglycerol are catalyzed by separate CTP-mediated kinase activities in Saccharomyces cerevisiae. Glycobiology 12, 555-62
3. Human dolichol kinase, a polytopic endoplasmic reticulum membrane protein with a cytoplasmically oriented CTP-binding site. J. Biol. Chem. 281, 31696-704
4. The Arabidopsis vitamin E pathway gene5-1 mutant reveals a critical role for phytol kinase in seed tocopherol biosynthesis. Plant Cell 18, 212-24
5. Temporal analysis reveals a key role for VTE5 in vitamin E biosynthesis in olive fruit during on-tree development. Front Plant Sci 6, 871