InterPro domain: IPR030662

This entry includes diphthine--ammonia ligases from eukaryotes and the uncharacterised protein MJ0570 from archaea. Diphthine--ammonia ligases are amidases that catalyse the last step of diphthamide biosynthesis using ammonium and ATP [ 1 , 2 ].

1. Chemogenomic approach identified yeast YLR143W as diphthamide synthetase. Proc. Natl. Acad. Sci. U.S.A. 109, 19983-7
2. The amidation step of diphthamide biosynthesis in yeast requires DPH6, a gene identified through mining the DPH1-DPH5 interaction network. PLoS Genet. 9, e1003334