InterPro domain: IPR030382

This entry represents a domain found in the Trm5/Tyw2-type methyltransferase. Trm5 and Tyw2 are S-adenosylmethionine-dependent methyltransferases involved in tRNA methylation [ 1 ].

Wybutosine synthesis is a multienzymatic process that comprises six sequential enzymatic reactions involving five enzymes. The initial step, the formation of m1G-37 (guanosine-37 methylated in the N1 position) in precursor tRNAPhe is catalysed by tRNA wybutosine-synthesising protein 5 (Trm5). Tyw2 catalyses the second step, consisting of the alpha-amino-alpha-carboxypropyl (acp) group being transferred from S-AdoMet to the side chain at the C7 position of 4-demethylwyosine (imG-14) to produce wybutosine-86 [ 2 ].

1. Biosynthesis of wyosine derivatives in tRNA: an ancient and highly diverse pathway in Archaea. Mol. Biol. Evol. 27, 2062-77
2. Biosynthesis of wybutosine, a hyper-modified nucleoside in eukaryotic phenylalanine tRNA. EMBO J. 25, 2142-54