InterPro domain: IPR029751

Ribosomal protein L25 is an RNA binding protein, that binds 5S rRNA [ 1 , 2 ]. Ribosomal protein L25 has a long-form and a short-form. The long-form is represented by the general stress protein Ctc of Bacillus subtilis, which has now been localised to ribosomes [ 3 ], and ribosomal protein TL5 of Thermus thermophilus. Ribosomal protein L25 of Escherichia coli and Haemophilus influenzae appear to be orthologous, but consist only of the N-terminal half of Ctc and TL5. Both short (L25-like) and full-length (CTC-like) members of this family bind the E-loop of bacterial 5S rRNA [ 4 , 5 ]. Eukaryotes contain a protein called L25, which is not homologous to bacterial L25, but rather to bacterial L23.

1. The NMR structure of Escherichia coli ribosomal protein L25 shows homology to general stress proteins and glutaminyl-tRNA synthetases. EMBO J. 17, 6377-84
2. The NMR structure of the 5S rRNA E-domain-protein L25 complex shows preformed and induced recognition. EMBO J. 18, 6508-21
3. The general stress protein Ctc of Bacillus subtilis is a ribosomal protein. J. Mol. Microbiol. Biotechnol. 4, 495-501
4. General stress protein CTC from Bacillus subtilis specifically binds to ribosomal 5S RNA. Biochemistry Mosc. 69, 607-11
5. Importance of the 5 S rRNA-binding ribosomal proteins for cell viability and translation in Escherichia coli. J. Mol. Biol. 366, 1199-208