InterPro domain: IPR027116

This entry includes negative cofactor 2 complex subunit alpha (also known as Bur6) and DNA polymerase epsilon subunit C (also known as Dpb3).

Bur6 is a component of the NC2 complex which represses RNA polymerase II transcription through binding to SPT15/TBP and thereby inhibiting the assembly of the preinitiation complex. The NC2 complex may also mediate transcriptional activation from TATA-driven promoters through association with SPT15/TBP [ 1 , 2 , 3 , 4 ].

Dpb3 is part of the DNA polymerase epsilon that participates in chromosomal DNA replication. It is essential for cell cycle progression [ 5 ].

1. Characterization of the basal inhibitor of class II transcription NC2 from Saccharomyces cerevisiae. Nucleic Acids Res. 24, 4450-5
2. The Dr1/DRAP1 heterodimer is a global repressor of transcription in vivo. Proc. Natl. Acad. Sci. U.S.A. 94, 820-5
3. Functional antagonism between RNA polymerase II holoenzyme and global negative regulator NC2 in vivo. Proc. Natl. Acad. Sci. U.S.A. 94, 3145-50
4. Direct stimulation of transcription by negative cofactor 2 (NC2) through TATA-binding protein (TBP). Proc. Natl. Acad. Sci. U.S.A. 99, 12727-32
5. Identification and cloning of two putative subunits of DNA polymerase epsilon in fission yeast. Nucleic Acids Res. 32, 4945-53