InterPro domain: IPR026846

This entry consists of the E3 SUMO-protein ligase Nse2 (also known as Mms21).

Nse2 is an E3 SUMO-protein ligase component of the SMC5-SMC6 complex [ 1 , 2 ]. Nse2 acts as an E3 ligase targeting several proteins for sumoylation and is required for efficient DNA repair and maintenance of genome stability [ 3 , 4 , 5 , 6 ]. Nse2 and SMC5 may also be required for sister chromatid cohesion during prometaphase and mitotic progression; this role is apparently independent of SMC6 [ 7 ]. Nse2 is necessary for normal cell cycle progression in Arabidopsis, where Nse2 mutation results in abnormal root development [ 8 ].

1. Novel essential DNA repair proteins Nse1 and Nse2 are subunits of the fission yeast Smc5-Smc6 complex. J. Biol. Chem. 278, 45460-7
2. Nse2, a component of the Smc5-6 complex, is a SUMO ligase required for the response to DNA damage. Mol. Cell. Biol. 25, 185-96
3. SUMO ligase activity of vertebrate Mms21/Nse2 is required for efficient DNA repair but not for Smc5/6 complex stability. DNA Repair (Amst.) 585, 2907-13
4. Human MMS21/NSE2 is a SUMO ligase required for DNA repair. Mol. Cell. Biol. 25, 7021-32
5. A SUMO ligase is part of a nuclear multiprotein complex that affects DNA repair and chromosomal organization. Proc. Natl. Acad. Sci. U.S.A. 102, 4777-82
6. The Nse2/Mms21 SUMO ligase of the Smc5/6 complex in the maintenance of genome stability. FEBS Lett.
7. SMC5 and MMS21 are required for chromosome cohesion and mitotic progression. Cell Cycle 8, 2211-8
8. The Arabidopsis SUMO E3 ligase AtMMS21, a homologue of NSE2/MMS21, regulates cell proliferation in the root. Plant J. 60, 666-78