InterPro domain: IPR025763
General Information
- Identifier IPR025763
- Description tRNA (guanine-N-7) methyltransferase catalytic subunit Trm8, eukaryote
- Number of genes 127
- Gene duplication stats Loading...
- Associated GO terms GO:0008176
Abstract
This entry represents the eukaryotic tRNA (guanine-N-7) methyltransferases catalytic subunit Trm8, and its homologue, such as METTL1 from humans [ 1 ].
In Saccharomyces cerevisiae, it forms the tRNA methyltransferase complex with Trm82 (WDR4 homologue in humans). This enzyme catalyses the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA, a modification required to maintain stability of tRNAs; its absence resulting in tRNA decay. Both the D-stem and T-stem structures of tRNAs are required for efficient methyltransferase activity [ 2 , 2 , 3 ]. The function of Trm8 is regulated by Trm82 [ 4 ].
1. Two proteins that form a complex are required for 7-methylguanosine modification of yeast tRNA. RNA 8, 1253-66
2. Rapid tRNA decay can result from lack of nonessential modifications. Mol. Cell 21, 87-96
3. RNA recognition mechanism of eukaryote tRNA (m7G46) methyltransferase (Trm8-Trm82 complex). FEBS Lett. 581, 1599-604
4. Structure of the yeast tRNA m7G methylation complex. Structure 16, 52-61