InterPro domain: IPR025640

This domain is found in bacteria, archaea and eukaryotes, and is approximately 50 amino acids in length. It contains an evolutionary conserved signature W-X-Y-X6-11-GPF-X4-M-X2-W-X3-GYF, the site of interaction with proline-rich peptides. Proteins containing this domain include RME-8 (Required for receptor-mediated endocytosis 8), a DNAJC13 protein. RME-8 was first identified as a protein that is required for endocytosis in Caenorhabditis elegans. It coordinates the activity of the WASH complex with the function of the retromer SNX dimer to control endosomal tubulation [ 1 ].

Proteins containing this domain also include Arabidopsis trithorax-related3 (Atxr3) and Tic56. Atxr3 is the major enzyme responsible for H3K4me3, which is critical for regulating gene expression and plant development [ 2 ]. Tic56 is an essential subunit of a 1-MDa protein complex at the inner chloroplast envelope membrane [ 3 ]. Tic56 also plays important roles in rRNA processing and chloroplast ribosome assembly [ 4 ].

1. RME-8 coordinates the activity of the WASH complex with the function of the retromer SNX dimer to control endosomal tubulation. J. Cell. Sci. 127, 2053-70
2. SET DOMAIN GROUP2 is the major histone H3 lysine [corrected] 4 trimethyltransferase in Arabidopsis. Proc. Natl. Acad. Sci. U.S.A. 107, 18557-62
3. Protein import-independent functions of Tic56, a component of the 1-MDa translocase at the inner chloroplast envelope membrane. Plant Signal Behav 12, e1284726
4. Importance of Translocon Subunit Tic56 for rRNA Processing and Chloroplast Ribosome Assembly. Plant Physiol. 172, 2429-2444