InterPro domain: IPR023370

General Information

  • Identifier IPR023370
  • Description TrmO-like, N-terminal domain
  • Number of genes 117
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Abstract

The following uncharacterised proteins have been shown to be evolutionary related and to contain a TrmO-like beta barrel structural domain domain:

  • Agrobacterium tumefaciens Ti plasmid protein virR.
  • Pseudomonas aeruginosa protein rcsF.
  • Archaeoglobus fulgidus hypothetical protein AF0241.
  • Archaeoglobus fulgidus hypothetical protein AF0433.
  • Methanococcus jannaschii hypothetical protein MJ1583.
  • Methanobacterium thermoautotrophicum hypothetical protein MTH1797.
  • Mammalian Nef-associated protein 1 (NAP1).

TrmO (also known as TsaA or YaeB) is a RNA methyltransferase responsible for N6-methylation of N6-threonylcarbamoyladenosine in tRNAs. It has a unique single-sheeted beta-barrel structure and represent a new category of AdoMet-dependent methyltransferases [ 1 ].

The crystal structure of Haemophilus influenzae TrmO has been solved. In addition to the N-terminal beta-barrel methyltransferase domain it has a C-terminal domain containing the conserved sequence motif DPRxxY [ 2 ].


1. Discovery of the β-barrel-type RNA methyltransferase responsible for N6-methylation of N6-threonylcarbamoyladenosine in tRNAs. Nucleic Acids Res. 42, 9350-65

Species distribution

Gene table

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