InterPro domain: IPR022998

Thiamine phosphate synthase (TPS) catalyzes the substitution of the pyrophosphate of 2-methyl-4-amino-5- hydroxymethylpyrimidine pyrophosphate by 4-methyl-5- (beta-hydroxyethyl)thiazole phosphate to yield thiamine phosphate in the thiamine biosynthesis pathway [ 1 ].

TenI, a protein from Bacillus subtilis that regulates the production of several extracellular enzymes by reducing alkaline protease production belongs to this group [ 2 ]. While TenI shows high sequence similarity with thiamin phosphate synthase [ 3 ], the purified protein has no thiamin phosphate synthase activity. Instead, it is a thiazole tautomerase [ 4 ].

This entry refers to a domain found in thiamine phosphate synthase, thiazole tautomerase and similar proteins.

1. Characterization of the Bacillus subtilis thiC operon involved in thiamine biosynthesis. J. Bacteriol. 179, 3030-5
2. Cloning and characterization of a pair of novel genes that regulate production of extracellular enzymes in Bacillus subtilis. J. Bacteriol. 173, 46-54
3. Structural characterization of the regulatory proteins TenA and TenI from Bacillus subtilis and identification of TenA as a thiaminase II. Biochemistry 44, 2319-29
4. A missing enzyme in thiamin thiazole biosynthesis: identification of TenI as a thiazole tautomerase. J. Am. Chem. Soc. 133, 9311-9