InterPro domain: IPR019407
General Information
- Identifier IPR019407
- Description Cytoplasmic tRNA 2-thiolation protein 2
- Number of genes 127
- Gene duplication stats Loading...
- Associated GO terms GO:0002098 GO:0000049 GO:0034227
Abstract
Cytoplasmic tRNA 2-thiolation protein 2 (also known as Ncs2/Tuc2 in budding yeasts) is responsible for 2-thiolation of mcm5S2U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln) [ 1 , 2 ]. Its fission yeast homologue, Ctu2 forms a complex with Ctu1 (Ncs6/Tuc1 homologue) and serves as a putative enzyme for the formation of 2-thiouridine [ 3 ].
1. Thio-modification of yeast cytosolic tRNA requires a ubiquitin-related system that resembles bacterial sulfur transfer systems. J. Biol. Chem. 283, 27469-76
2. The conserved Wobble uridine tRNA thiolase Ctu1-Ctu2 is required to maintain genome integrity. Proc. Natl. Acad. Sci. U.S.A. 105, 5459-64
3. Mechanistic characterization of the sulfur-relay system for eukaryotic 2-thiouridine biogenesis at tRNA wobble positions. Nucleic Acids Res. 37, 1335-52