InterPro domain: IPR018464
General Information
- Identifier IPR018464
- Description Centromere protein O
- Number of genes 115
- Gene duplication stats Loading...
- Associated GO terms GO:0034508 GO:0000776
Abstract
This entry represents centromere protein O (CENP-O) and its homologues in yeasts, Mcm21 and Mal2.
In humans, centromere protein O (CENP-O) is a component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation [ 1 ]. CENP-O mediates the attachment of the centromere to the mitotic spindle by forming essential interactions between the microtubule-associated outer kinetochore proteins and the centromere-associated inner kinetochore proteins. CENP-O modulates the kinetochore-bound levels of NDC80 complex [ 2 ]. It may be involved in incorporation of newly synthesized CENP-A into centromeres via its interaction with the CENPA-NAC complex [ 3 ].
In Saccharomyces cerevisiae, Mcm21 is a component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p), which links kinetochore subunits with subunits bound to microtubules during kinetochore assembly [ 4 , 5 ].
In Schizosaccharomyces pombe, Mal2 is a component of the Sim4 complex, which is required for loading the DASH complex onto the kinetochore via interaction with Dad1 [ 6 ]. It plays a role in the maintenance of core chromatin structure and kinetochore function [ 7 ].
1. The human CENP-A centromeric nucleosome-associated complex. Nat. Cell Biol. 8, 458-69
2. The CENP-A NAC/CAD kinetochore complex controls chromosome congression and spindle bipolarity. EMBO J. 26, 5033-47
3. The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres. Nat. Cell Biol. 8, 446-57
4. Hierarchical assembly of the budding yeast kinetochore from multiple subcomplexes. Genes Dev. 17, 2902-21
5. CENP-T proteins are conserved centromere receptors of the Ndc80 complex. Nat. Cell Biol. 14, 604-13
6. The mal2p protein is an essential component of the fission yeast centromere. Mol. Cell. Biol. 22, 7168-83
7. Molecular analysis of kinetochore architecture in fission yeast. EMBO J. 24, 2919-30