InterPro domain: IPR017736

O-Glycosyl hydrolases ( 3.2.1. ) are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families [ 1 , 2 ]. This classification is available on the CAZy (CArbohydrate-Active EnZymes) website.

Glycoside hydrolase family 1 GH1 comprises enzymes with a number of known activities; beta-glucosidase ( 3.2.1.21 ); beta-galactosidase ( 3.2.1.23 ); 6-phospho-beta-galactosidase ( 3.2.1.85 ); 6-phospho-beta-glucosidase ( 3.2.1.86 ); lactase-phlorizin hydrolase ( 3.2.1.62 ), ( 3.2.1.108 ); beta-mannosidase ( 3.2.1.25 ); myrosinase ( 3.2.1.147 ).

This enzyme represents beta-glucosidase, which catalyses the hydrolysis of terminal, non-reducing beta-D-glucose residues with release of beta-D-glucose [ 3 , 4 ]. It has a wide substrate specificity for beta-D-glucosides.

1. Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases. Proc. Natl. Acad. Sci. U.S.A. 92, 7090-4
2. Structures and mechanisms of glycosyl hydrolases. Structure 3, 853-9
3. Crystal structure of beta-glucosidase A from Bacillus polymyxa: insights into the catalytic activity in family 1 glycosyl hydrolases. J. Mol. Biol. 275, 491-502
4. Cloning and characterization of the glucooligosaccharide catabolic pathway beta-glucan glucohydrolase and cellobiose phosphorylase in the marine hyperthermophile Thermotoga neapolitana. J. Bacteriol. 182, 5172-9