InterPro domain: IPR014808

Dna2 and its plant homologue JHS1 are DNA replication factors with single-stranded DNA-dependent ATPase, ATP-dependent nuclease, (5'-flap endonuclease) and helicase activities. It is required for Okazaki fragment processing and is involved in DNA repair pathways [ 1 , 2 ]. The helicase activity is weak and its function remains unclear [ 3 , 4 , 5 , 6 ].

This entry represents N-terminal domain of the DNA replication factor Dna2.

1. Genetic analyses of Schizosaccharomyces pombe dna2(+) reveal that dna2 plays an essential role in Okazaki fragment metabolism. Genetics 155, 1055-67
2. Biochemical analyses indicate that binding and cleavage specificities define the ordered processing of human Okazaki fragments by Dna2 and FEN1. Nucleic Acids Res. 40, 6774-86
3. Identification of the Xenopus laevis homolog of Saccharomyces cerevisiae DNA2 and its role in DNA replication. J. Biol. Chem. 275, 1615-24
4. Isolation of human Dna2 endonuclease and characterization of its enzymatic properties. Nucleic Acids Res. 34, 1854-64
5. Biochemical analysis of human Dna2. Nucleic Acids Res. 34, 1865-75
6. Acetylation of Dna2 endonuclease/helicase and flap endonuclease 1 by p300 promotes DNA stability by creating long flap intermediates. J. Biol. Chem. 285, 4398-404