InterPro domain: IPR014608

This entry represents eukaryotic ATP-citrate synthase (ACLY) ( 2.3.3.8 ), also known as ATP citrate lyase. In bacteria and archaea, ATP citrate synthase is composed of two distinct subunits [ 1 ]. In eukaryotes, ATP citrate synthase is a homotetramer of a single large polypeptide, and is used to produce cytosolic acetyl-CoA from mitochondrial produced citrate [ 2 , 3 , 4 , 5 ]. Acetyl-CoA is the building block for de novo lipid synthesis. As such, ACLY couples energy metabolism with fatty acids synthesis and plays a critical role in supporting cell growth [ 6 ].

1. Both subunits of ATP-citrate lyase from Chlorobium tepidum contribute to catalytic activity. J. Bacteriol. 188, 6544-52
2. ATP citrate lyase is an important component of cell growth and transformation. Oncogene 24, 6314-22
3. Cloning and expression of a human ATP-citrate lyase cDNA. Eur. J. Biochem. 204, 491-9
4. The fungal acl1 and acl2 genes encode two polypeptides with homology to the N- and C-terminal parts of the animal ATP citrate lyase polypeptide. Curr. Genet. 37, 189-93
5. Rat ATP citrate-lyase. Molecular cloning and sequence analysis of a full-length cDNA and mRNA abundance as a function of diet, organ, and age. J. Biol. Chem. 265, 1430-5
6. Acetylation stabilizes ATP-citrate lyase to promote lipid biosynthesis and tumor growth. Mol. Cell 51, 506-18