InterPro domain: IPR013893

This entry represents Rpp40 from eukaryotes. Human RNase P is composed of a singular protein Pop1 and three subcomplexes, the Rpp20-Rpp25 heterodimer, Pop5-Rpp14-(Rpp30)2-Rpp40 heteropentamer, and Rpp21-Rpp29-Rpp38 heterotrimer [ 1 ].

Ribonuclease P (Rnp) is a ubiquitous ribozyme that catalyzes a Mg2 -dependent hydrolysis to remove the 5'-leader sequence of precursor tRNA (pre-tRNA) in all three domains of life [ 2 ]. In bacteria, the catalytic RNA (typically ~120kDa) is aided by a small protein cofactor (~14kDa) [ 3 ]. Archaeal and eukaryote RNase P consist of a single RNA and archaeal RNase P has four or five proteins, while eukaryotic RNase P consists of 9 or 10 proteins. Eukaryotic and archaeal RNase P RNAs cooperatively function with protein subunits in catalysis [ 4 ].

1. Rpp14 and Rpp29, two protein subunits of human ribonuclease P. RNA 5, 153-7
2. The nucleotide sequence of chromosome I from Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. U.S.A. 92, 3809-13
3. Chance and necessity in the evolution of RNase P. RNA 24, 1-5