InterPro domain: IPR013535

The PUL (after PLAP, UFD3 and lub1) domain is a predicted predominantly alpha helical globular domain found in eukaryotes. It is found in association with either WD repeats (see PDOC00574 ) and the PFU domain (see PDOC51394 ) or PPPDE and thioredoxin (see PDOC00172 ) domains. The PUL domain is a protein-protein interaction domain [ 1 , 2 ].

Some proteins known to contain a PUL domain are listed below:

• Saccharomyces cerevisiae DOA1 (UFD3, ZZZ4), involved in ubiquitin conjugation pathway. DOA1 participates in the regulation of the ubiquitin conjugation pathway involving CDC48 by hindering multiubiquitination of substrates at the CDC48 chaperone.
• Schizosaccharomyces pombe ubiquitin homeostasis protein lub1, acts as a negative regulator of vacuole-dependent ubiquitin degradation.
• Mammalian phospholipase A-2-activating protein (PLA2P, PLAA), the homologue of DOA1. PLA2P plays an important role in the regulation of specific inflammatory disease processes.

1. Novel predicted peptidases with a potential role in the ubiquitin signaling pathway. Cell Cycle 3, 1440-50
2. Doa1 is a Cdc48 adapter that possesses a novel ubiquitin binding domain. Mol. Cell. Biol. 26, 822-30