InterPro domain: IPR013183

This entry includes Hsk3, Hos3 and Golgin-45.

Hsk3 is a subunit of the DASH complex, which is a ~10 subunit microtubule-binding complex that is transferred to the kinetochore prior to mitosis [ 1 ]. In Saccharomyces cerevisiae DASH forms both rings and spiral structures on microtubules in vitro [ 2 , 3 ]. This family also includes several higher eukaryotic proteins. However, other DASH subunits do not appear to be conserved in higher eukaryotes.

Golgin-45 is required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface. It interacts with GORASP2 and with the GTP-bound form of RAB2, but not with other Golgi Rab proteins [ 4 ].

Hos3 (High osmolarity sensitivity protein 3) may play a role in the progression of mitosis in an environment of high osmotic stress [ 5 ].

1. The mitotic spindle is required for loading of the DASH complex onto the kinetochore. Genes Dev. 16, 183-97
2. The yeast DASH complex forms closed rings on microtubules. Nat. Struct. Mol. Biol. 12, 138-43
3. Formation of a dynamic kinetochore- microtubule interface through assembly of the Dam1 ring complex. Mol. Cell 17, 277-90
4. A GRASP55-rab2 effector complex linking Golgi structure to membrane traffic. J. Cell Biol. 155, 877-83
5. Identification and characterization of a novel gene, hos3+, the function of which is necessary for growth under high osmotic stress in fission yeast. Biosci. Biotechnol. Biochem. 64, 1099-102